Transfer Membranes

Transfer Membranes

Overview

Cobetter PVDF transfer membranes feature high sensitivity and low background in Western blotting. The PVDF membrane is hydrophobic and needs to be prewetted by methanol. We offer pre-cut membrane sheets as well as coils. And available in 0.45μm for most Western blotting application and 0.2μm for immunoblotting of low MW proteins <20kDa.

Overview

Western Blotting is a method to analyze and quantify proteins. The protein is separated by gel electrophoresis to a solid phase support (PVDF membrane). Antibodies can generate antigen-antibody immune reactions with the target proteins attached to the PVDF membrane. The western blotting technology is to identify and semi quantitatively analyze a specific protein.

The western blotting protocol can be roughly divided into the following 7 steps: cell culture, protein extraction, concentration and exchange, SDS-PAGE electrophoresis, membrane transfer, blocking and antibody incubation, and protein detection.



Cobetter Hydrophilicity Applications Thickness  Available Sizes

PVDF, 0.45µm

Hydrophobic

Optimized for Western blotting

140 μm

265mm*3.75m;

150mm*150mm;

200mm*200mm;

84mm*70mm

PVDF, 0.2µm

Hydrophobic

For protein sequencing and immunoblotting of low molecular weight proteins

130 μm

265mm*3.75m;

150mm*150mm;

200mm*200mm;

84mm*70mm

 

Membrane Performance

Notes

· Wet the PVDF membrane in methanol for 15 seconds.

· For samples containing small peptides, the equilibrium time of the gel in the transfer buffer should be limited to 10 minutes.

· Prevent bubbles from entering into the filter paper, gel or membrane.

· When the target protein is less than or equal to 20kDa, 0.22um PVDF membrane can be used to obtain the maximum retention.

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