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Gene therapy is the process of introducing exogenous normal genes into target cells to correct or compensate for diseases caused by defects and abnormal genes, to achieve therapeutic goals. Gene therapy bring new hope for the treatment of genetic diseases, cancer and infectious diseases.
Virus has a natural ability to transport genetic material into cells. Gene therapy mainly uses viral vectors to directly introduce complete genes into the patient's body (in vivo) to reconstruct or modify harmful gene variants.
Plasmids (pDNA) are circular DNA molecules present in bacteria. They can replicate independently from bacterial DNA, which makes them very suitable as carriers for genetic engineering. Purified plasmids can be used for the production of adeno-associated virus (AAV) and lentiviral vectors.
pDNA Production Process Flow
