Ultrafiltration & Concentration

Home Applications Biopharmaceutical Manufacturing Monoclonal Antibody Ultrafiltration & Concentration

Ultrafiltration is an important step to separate, purify and concentrate protein solutions in biopharmaceutical manufacturing, improving production capacity and recovery rate. The retention capacity of ultrafiltration membranes are characterized by the molecular weight cut-off (MWCO) of the target protein, typically ranging from 1kDa to 1000kDa.

Determine the MWCO of Ultrafiltration Membrane

To have the target macromolecule permeate the membrane, generally choose an ultrafiltration membrane with a MWCO that is 3-5 times bigger than the MW of target macromolecule.
To have the target macromolecule to be retained, generally choose an ultrafiltration membrane with a MWCO that is 3-5 times smaller than the MW of target macromolecule.

Choose the Right TFF Cassettes

	Polyethersulfone（PES）	Regenerated Cellulose（RC）
Features	pH range: 1-14 High flux, very low protein binding	Natural Hydrophilic Resistant to organic solvents Suitable for high concentration formulation
MWCO	1, 3, 5, 8, 10, 30, 50, 100, 300, 500, 1000 kDa	1, 2, 3, 5, 8, 10, 30, 50, 100, 300 kDa
Typical Applications	Oligonucleotide, Peptide, Proteins, Growth factor, FVIII，Recombinant proteins，mAb, Vaccines, Viral vectors	Peptide, Insulin, Growth factor, Recombinant proteins，mAb, Collagen, LNP, Exosome, Viral vectors
Filtration Area	100cm², 200 cm², 0.11 m², 0.46m², 0.5 m², 2.33 m², 2.5 m²

Choose the Right Hollow Fiber TFF

	Fiber ID	Effective Length	Batch Volumes	Feed/Retentate Connectors	Permeate Connectors	MWCO
Mini	0.5mm 1.0mm	30cm 60cm	<600mL	Female Luer	Female Luer	3 kDa 5 kDa 10 kDa 30 kDa 50 kDa 100 kDa 300 kDa 500 kDa 750 kDa
Minilab	0.5mm 1.0mm	30cm 60cm	<2L	Female Luer	Female Luer
Lab	0.5mm 1.0mm	30cm 60cm	2L-16L	0.5'' TC	3/16'' HB
Pilot	0.5mm 1.0mm	30cm 60cm 110cm	15L-80L	1.5'' TC	0.5'' TC
Process	0.5mm 1.0mm	30cm 60cm 110cm	75L-800L	1.5'' TC	1.0'' TC
Maxi	0.5mm 1.0mm	30cm 60cm 110cm	800L-1500L	2.0'' TC	1.5'' TC

TFF Cassettes

Available in PES and RC ultrafiltration membrane, with a wide range of MWCO. Low protein binding, easy linear scale-up from lab to process and production.
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Hollow Fiber Filters

Modified hydrophilic PES hollow fiber, low protein binding, high flow rate, and high loading capacity. Used for cell harvest, clarification and concentration. Easy to install and linear scale-up.
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