|Connections||Inlet||Female Luer Lock|
|Outlet||Male Luer Slip|
|Physical Properties||Pore Size||0.45+0.22 μm, 0.8+0.45 μm|
|Maximum Operating Pressure||
|pH Range||1.0 – 14.0|
|Sterilization Method||Autoclave at 121ºC (130ºC max) for 30 minutes|
Cobetter high flux syringe filters are constructed of polypropylene housing with a prefiltration membrane with pore sizes ranging from 0.45μm to 0.8μm. This construction prevents the build-up of back pressure typically caused by the blocking of an unprotected membrane.
Cobetter high flux syringe filters can process three times more sample volume than standard syringe filters.
· 13 and 33 mm diameter syringe filters
· Double-layer membrane with graduated pore size for excellent loading capacity with fast flow rates
· Individually packed, Eliminates sample contamination
· Requires less hand pressure
· Polyethersulfone (PES) for serum, plasma and tissue culture
· Hydrophilic Polyvinylidene difluoride (PVDF) for biological solutions
· Routine sample preparation
· Environmental samples
Select by Volume
|Sample Volume||Filter Diameter||Filtration Area||Retention Volume|
|< 10mL||13mm||0.72 cm²||< 25mL|
|10mL to 100mL||25mm||3.4 cm²||< 100mL|
|100mL to 200mL||33mm||4.5 cm²||< 125mL|
How to use a syringe filter?
1. Choose the right type of membrane material based on the properties of your sample. Be careful of the chemical compatibility to avoid extractables and leachables. Read this article about How to choose a syringe filter.
2. Choose the right pore size and diameter:
· 1-10ml samples: 13mm syringe filter is recommended, the hold-up volume is less than 25μl.
· 5-100ml samples: 25mm syringe filter is recommended, the hold-up volume is less than 100μl.
· 10-200ml samples: 33mm syringe filter is recommended, the hold-up volume is less than 125μl.
3. Load the sample into the syringe.
4. Attach the filter to the luer-lock of the syringe with a twisting motion. Make sure it is securely attached.
5. Filter the sample. Hold the syringe and filter vertically, and gently press the plunger to push the sample through the filter. Take care not to apply too much force or you may damage the filter.
6. Discard the used filter and repeat for the next sample.
How to sterilize syringe filters?
Only autoclavable syringe filters can be autoclaved. They can withstand autoclaving at 121°C (up to a maximum of 130°C) for 30 minutes, and this process can be repeated up to 20 times. However, we recommend using it only once for optimal performance after autoclaving.
Gamma irradiation involves exposing the filters to high-energy gamma rays, which can kill microorganisms such as bacteria, viruses and fungi. This method is typically used for large-scale sterilization in a relatively short time. Cobetter sterile syringe filters are gamma-irradiated and individually packed.
Additionally, be sure to handle the filters with clean gloves or use them on a clean bench to avoid introducing contaminants while using.