COBETTER 0.2μm Hydrophobic PTFE Syringe Filters for Air/Gas Venting
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| Connections | Inlet | Female Luer Lock | |||
| Outlet | Male Luer Slip | ||||
| Materials | Housing |
PP | |||
| Membrane | PTFE | ||||
| Wettability | Hydrophobic | ||||
| Physical Properties | Pore Size | 0.2 μm | |||
| Filter Diameter |
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| Filtration Area |
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| Maximum Operating Pressure |
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| Maximum Operating Temperature | 50°C | ||||
| pH Range | 1.0 – 14.0 | ||||
| Compliance | Bacterial Endotoxins | < 0.25 EU/mL as determined by the LAL test | |||
| Biosafety | All materials of this filter meets USP Biological Reactivity tests <88> for plastics Class VI | ||||
| Sterility | Delivery Condition | Non-sterile, and Autoclavable | |||
| Sterilization Method | Sterile by Gamma Irradiation | ||||
| Package | Pack Size | 100pcs | |||
| Packaging | Individually packed |
Features
· High retention rates
· Low-pressure differential
· Efficient mycoplasma, bacteria and particulate retention
Applications
· Vent Filters for fermentors, tanks, etc.
· Sterile-grade filtration for compressed air, oxygen, nitrogen, etc.
Select by Volume
| Sample Volume | Filter Diameter | Filtration Area | Retention Volume |
| < 10mL | 13mm | 0.72 cm² | < 25mL |
| 10mL to 100mL | 25mm | 3.4 cm² | < 100mL |
| 100mL to 200mL | 33mm | 4.5 cm² | < 125mL |
How to use a syringe filter?
1. Choose the right type of membrane material based on the properties of your sample. Be careful of the chemical compatibility to avoid extractables and leachables. Read this article about How to choose a syringe filter.
2. Choose the right pore size and diameter:
· 1-10ml samples: 13mm syringe filter is recommended, the hold-up volume is less than 25μl.
· 5-100ml samples: 25mm syringe filter is recommended, the hold-up volume is less than 100μl.
· 10-200ml samples: 33mm syringe filter is recommended, the hold-up volume is less than 125μl.
3. Load the sample into the syringe.
4. Attach the filter to the luer-lock of the syringe with a twisting motion. Make sure it is securely attached.
5. Filter the sample. Hold the syringe and filter vertically, and gently press the plunger to push the sample through the filter. Take care not to apply too much force or you may damage the filter.
6. Discard the used filter and repeat for the next sample.
How to sterilize syringe filters?
Autoclaving
Only autoclavable syringe filters can be autoclaved. They can withstand autoclaving at 121°C (up to a maximum of 130°C) for 30 minutes, and this process can be repeated up to 20 times. However, we recommend using it only once for optimal performance after autoclaving.
Gamma Irradiation
Gamma irradiation involves exposing the filters to high-energy gamma rays, which can kill microorganisms such as bacteria, viruses and fungi. This method is typically used for large-scale sterilization in a relatively short time. Cobetter sterile syringe filters are gamma-irradiated and individually packed.
Additionally, be sure to handle the filters with clean gloves or use them on a clean bench to avoid introducing contaminants while using.