COBETTER 1mm Pilot Hollow Fiber TFF, mPES

In stock
Part No. HFEPI01001030P
Length
MWCO
Connections Feed/Retentate connectors 1.5'' TC
Permeate connector 0.5'' TC
Materials Membrane material mPES
Housing polysulfone
End Cap
white-polysulfone
Seals (internal) epoxy resin
Physical Properties MWCO 100 kDa, 300 kDa, 500 kDa, 750 kDa
Fiber ID 1.0 mm
No. Of Fibers 160
Effective Length
30mm 60mm 110mm
Dimensions(mm*mm)
33*361 33*661 33*1161
Effective membrane area
0.15m² 0.30m² 0.55m²
Recommended batch volume per module
<15L <30L <50L
Performance Corssflow rate @2000sec-[mL/min] 1,884
Corssflow rate @6000sec-[mL/min] 5,652
Sterility Delivery Condition
Nonsterile
Package Pack Size 1pcs
Packaging Individually packed in PE bag

 

By offering various lengths, membrane filtration areas, and module diameters, Cobetter hollow fiber modules can facilitate a smooth and predictable scale-up from laboratory to pilot-scale and eventually manufacturing-scale. Available from Mini, Minilab, Lab, Pilot, Pilot+, Process and Max.

Cobetter hollow fiber modules are made of modified polyethersulfone membranes(m-PES), which have high flow flux and low protein binding. They are easy to install, clean and sanitize, and are widely used in cell harvest, clarification, perfusion, diafiltration and concentration in upstream and downstream bioprocessing.

Compare with TFF Cassette’s tortuous and complicated flow path, hollow fiber filtration has a linear flow path which creates a smooth flow for more gentle separation.

Features

· Easy to scale-up

· Easy to setup

· High process flux

· Low protein binding

· Cost-effective and time-saving

· Don’t require any hardware installation

Applications

· Clarification of cells and bacterial from fermentation broth

· Clarification of cell lysates or cell debris

· Concentration and diafiltration of proteins

· Purification, concentration and diafiltration of viral vector

· Perfusion applications

Choose Fiber ID

· 0.5 mm ID fibers are mostly used for general applications.

· 1.0 mm ID fibers are mostly used in materials with high cell density, high solid content, or high viscosity, such as glycerol or sucrose, or if either condition is accompanied by cold-room processing(i.e., 4°C)

How Does Hollow Fiber Filber Work

Check the hollow fiber filter mechanism diagram below.

How to use a hollow fiber membrane filter?

Module Preparation

For lab and pilot applications, you will need a pump, a feed tank, a permeate collection tank, pressure gauges and valves and suitable tubing and connectors.

Cobetter hollow fiber modules made with hydrophilic modified PES membrane contain glycerin to preserve the pore structure. Glycerine is water soluble and easy to rinse out.

  1. Rinse out glycerine with DI water with an inlet pressure of 2-5 psi, the flushing volume should be greater than 20L/.
  2. Drain module and perform module integrity test.

 Note: If sanitization is necessary, use 0.1N NaOH solution as a disinfectant.

 

Module Integrity Test

All hollow fiber modules are integrity tested at our site. However, Cobetter highly recommends an integrity test before use. Cobetter recommends two test methods, one of which may be applied according to actual operating conditions.  

  1. Diffusion Flow Method
  2. Pressure Retaining Method

 

Processing Buffer Equilibrium

The purpose of flushing hollow fiber modules with buffer is to ensure that the pH and salt ion conditions of the system are as similar as possible to the initial state of the sample, so as to reduce pollution and product loss.

 

Cleaning after Use

The hollow fiber modules must be cleaned before storage and reused by flushing with buffer or DI water, and then washed with appropriate sanitizing agent. 0.5M NaOH solution may be used to circulate for 30 min at 20-40°C.

 

Hollow Fiber Storage

The hollow fiber modules may be stored in 0.1N NaOH solution. For long-term storage between 1-12 months, it is recommended to be stored under refrigeration at 4°C.