0.5mm Pilot+ Hollow Fiber TFF, mPES

In stock
Part No. HFEPP01000530P

By offering various lengths, membrane filtration areas, and module diameters, Cobetter hollow fiber modules can facilitate a smooth and predictable scale-up from laboratory to pilot-scale and eventually manufacturing-scale. Available from Mini, Minilab, Lab, Pilot, Pilot+, Process and Max.

Cobetter hollow fiber modules are made of modified polyethersulfone membranes(m-PES), which have high flow flux and low protein binding. They are easy to install, clean and sanitize, and are widely used in cell harvest, clarification, perfusion, diafiltration and concentration in upstream and downstream bioprocessing.

Compare with TFF Cassette’s tortuous and complicated flow path, hollow fiber filtration has a linear flow path which creates a smooth flow for more gentle separation.


Materials Feed/Retentate connectors 1.5'' TC

Permeate connector 0.5'' TC

Membrane material mPES

Housing polysulfone

End Cap

Seals (internal) epoxy resin
Physical Properties MWCO 100 kDa, 300 kDa, 500 kDa, 750 kDa

Fiber ID 0.5 mm

No. Of Fibers 500

Effective Length
30mm 60mm 110mm

33*361 33*661 33*1161

Effective membrane area
0.24m² 0.47m² 0.86m²

Recommended batch volume per module
<15L <50L <80L

Corssflow rate @2000sec-[mL/min] 736

Corssflow rate @6000sec-[mL/min] 2206
Sterility Delivery Condition
Package Pack Size 1pcs

Packaging Individually packed in PE bag


  • High flux rates, high filtration capacity
  • Modified hydrophilic PES hollow fiber membrane provides low protein binding, less membrane fouling and easy cleaning
  • As a complete device without additional assembly or device holder, quick installation and operation
  • Regenerated by chemical wash with 0.5M NaOH solution
  • Simple and reliable linear amplified scale-up


  • Purification, concentration and diafiltration of vaccine 
  • Purification, concentration and diafiltration of viral vector
  • Clarification of cells and bacterial in fermentation broth
  • Recycling and washing of cells and bacteria
  • Concentration and diafiltration of protein

Choose Fiber ID

  • 0.5 mm ID fibers are mostly used for general applications.
  • 1.0 mm ID fibers are mostly used in materials with high cell density, high solid content, or high viscosity, such as glycerol or sucrose, or if either condition is accompanied by cold-room processing(i.e., 4°C)

How Does Hollow Fiber Filber Work

Check the hollow fiber filter mechanism diagram below.

How to use a hollow fiber membrane filter?

Module Preparation

For lab and pilot applications, you will need a pump, a feed tank, a permeate collection tank, pressure gauges and valves and suitable tubing and connectors.

Cobetter hollow fiber modules made with hydrophilic modified PES membrane contain glycerin to preserve the pore structure. Glycerine is water soluble and easy to rinse out.

  1. Rinse out glycerine with DI water with an inlet pressure of 2-5 psi, the flushing volume should be greater than 20L/.
  2. Drain module and perform module integrity test.

 Note: If sanitization is necessary, use 0.1N NaOH solution as a disinfectant.


Module Integrity Test

All hollow fiber modules are integrity tested at our site. However, Cobetter highly recommends an integrity test before use. Cobetter recommends two test methods, one of which may be applied according to actual operating conditions.  

  1. Diffusion Flow Method
  2. Pressure Retaining Method


Processing Buffer Equilibrium

The purpose of flushing hollow fiber modules with buffer is to ensure that the pH and salt ion conditions of the system are as similar as possible to the initial state of the sample, so as to reduce pollution and product loss.


Cleaning after Use

The hollow fiber modules must be cleaned before storage and reused by flushing with buffer or DI water, and then washed with appropriate sanitizing agent. 0.5M NaOH solution may be used to circulate for 30 min at 20-40°C.


Hollow Fiber Storage

The hollow fiber modules may be stored in 0.1N NaOH solution. For long-term storage between 1-12 months, it is recommended to be stored under refrigeration at 4°C.