COBETTER 0.5mm Pilot Hollow Fiber TFF, mPES
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|Connections||Feed/Retentate connectors||1.5'' TC|
|Permeate connector||0.5'' TC|
|Seals (internal)||epoxy resin|
|Physical Properties||MWCO||100 kDa, 300 kDa, 500 kDa, 750 kDa|
|Fiber ID||0.5 mm|
|No. Of Fibers||320|
|Effective membrane area||
|Recommended batch volume per module||
|Performance||Corssflow rate @2000sec-[mL/min]||471|
|Corssflow rate @6000sec-[mL/min]||1,413|
|Packaging||Individually packed in PE bag|
By offering various lengths, membrane filtration areas, and module diameters, Cobetter hollow fiber modules can facilitate a smooth and predictable scale-up from laboratory to pilot-scale and eventually manufacturing-scale. Available from Mini, Minilab, Lab, Pilot, Pilot+, Process and Max.
Cobetter hollow fiber modules are made of modified polyethersulfone membranes(m-PES), which have high flow flux and low protein binding. They are easy to install, clean and sanitize, and are widely used in cell harvest, clarification, perfusion, diafiltration and concentration in upstream and downstream bioprocessing.
Compare with TFF Cassette’s tortuous and complicated flow path, hollow fiber filtration has a linear flow path which creates a smooth flow for more gentle separation.
· Easy to scale-up
· Easy to setup
· High process flux
· Low protein binding
· Cost-effective and time-saving
· Don’t require any hardware installation
· Clarification of cells and bacterial from fermentation broth
· Clarification of cell lysates or cell debris
· Concentration and diafiltration of proteins
· Purification, concentration and diafiltration of viral vector
· Perfusion applications
Choose Fiber ID
· 0.5 mm ID fibers are mostly used for general applications.
· 1.0 mm ID fibers are mostly used in materials with high cell density, high solid content, or high viscosity, such as glycerol or sucrose, or if either condition is accompanied by cold-room processing(i.e., 4°C)
How Does Hollow Fiber Filber Work
Check the hollow fiber filter mechanism diagram below.
How to use a hollow fiber membrane filter?
For lab and pilot applications, you will need a pump, a feed tank, a permeate collection tank, pressure gauges and valves and suitable tubing and connectors.
Cobetter hollow fiber modules made with hydrophilic modified PES membrane contain glycerin to preserve the pore structure. Glycerine is water soluble and easy to rinse out.
- Rinse out glycerine with DI water with an inlet pressure of 2-5 psi, the flushing volume should be greater than 20L/m².
- Drain module and perform module integrity test.
Note: If sanitization is necessary, use 0.1N NaOH solution as a disinfectant.
Module Integrity Test
All hollow fiber modules are integrity tested at our site. However, Cobetter highly recommends an integrity test before use. Cobetter recommends two test methods, one of which may be applied according to actual operating conditions.
- Diffusion Flow Method
- Pressure Retaining Method
Processing Buffer Equilibrium
The purpose of flushing hollow fiber modules with buffer is to ensure that the pH and salt ion conditions of the system are as similar as possible to the initial state of the sample, so as to reduce pollution and product loss.
Cleaning after Use
The hollow fiber modules must be cleaned before storage and reused by flushing with buffer or DI water, and then washed with appropriate sanitizing agent. 0.5M NaOH solution may be used to circulate for 30 min at 20-40°C.
Hollow Fiber Storage
The hollow fiber modules may be stored in 0.1N NaOH solution. For long-term storage between 1-12 months, it is recommended to be stored under refrigeration at 4°C.