|Connections||Inlet||Female Luer Lock|
|Outlet||Male Luer Slip|
|Physical Properties||Pore Size||0.2 μm(prefilter)+0.1 μm|
|Maximum Operating Pressure||
|pH Range||1.0 – 14.0|
|Sterilization Method||Gamma Irradiation|
|Packaging||Single blister packed|
Cobetter sterile PES syringe filters are individually packed and made of two layers of asymmetric polyethersulfone (PES) membrane. The first layer is a prefilter with larger pore size which makes the syringe filter achieve high throughput and fast flow rate. The housing is made of premium polypropylene which has a wide chemical compatibility. The Over molded design ensures higher operating pressures. Luer lock inlet/outlet offers leak-tight syringe connection.
· Available in pore size 0.2μm(prefilter)+0.1μm
· Graduated pore sizes for high throughput
· Fast flow rate and low protein binding
· 0.1μm for mycoplasma filtration
· 0.2μm for sterilization of tissue culture media, protein solutions or aqueous solutions
· 0.45μm for clarification and cell removal
Select by Volume
|Sample Volume||Filter Diameter||Filtration Area||Retention Volume|
|< 10mL||13mm||0.72 cm²||< 25mL|
|10mL to 100mL||25mm||3.4 cm²||< 100mL|
|100mL to 200mL||33mm||4.5 cm²||< 125mL|
Sterile Syringe Filters
Cobetter sterile syringe filters are ideal for sterile filtration of cell culture media with a high flow rate and low protein adsorption.
· 0.1 µm for mycoplasma filtration
· 0.22 µm for sterile filtration
· 0.45 µm for clarification
· 0.8 µm for particle and yeast cell removal
Cobetter Sterile PES Syringe Filters with High Flow Rate
How to use a syringe filter?
1. Choose the right type of membrane material based on the properties of your sample. Be careful of the chemical compatibility to avoid extractables and leachables. Read this article about How to choose a syringe filter.
2. Choose the right pore size and diameter:
· 1-10ml samples: 13mm syringe filter is recommended, the hold-up volume is less than 25μl.
· 5-100ml samples: 25mm syringe filter is recommended, the hold-up volume is less than 100μl.
· 10-200ml samples: 33mm syringe filter is recommended, the hold-up volume is less than 125μl.
3. Load the sample into the syringe.
4. Attach the filter to the luer-lock of the syringe with a twisting motion. Make sure it is securely attached.
5. Filter the sample. Hold the syringe and filter vertically, and gently press the plunger to push the sample through the filter. Take care not to apply too much force or you may damage the filter.
6. Discard the used filter and repeat for the next sample.
How to sterilize syringe filters?
Only autoclavable syringe filters can be autoclaved. They can withstand autoclaving at 121°C (up to a maximum of 130°C) for 30 minutes, and this process can be repeated up to 20 times. However, we recommend using it only once for optimal performance after autoclaving.
Gamma irradiation involves exposing the filters to high-energy gamma rays, which can kill microorganisms such as bacteria, viruses and fungi. This method is typically used for large-scale sterilization in a relatively short time. Cobetter sterile syringe filters are gamma-irradiated and individually packed.
Additionally, be sure to handle the filters with clean gloves or use them on a clean bench to avoid introducing contaminants while using.