COBETTER Briscale® 4mL Ultrafiltration 10 kDa Centrifugal Filter with Regenerated Cellulose RC Membrane 15/pk

In stock
Part No. ULRC0100040P
Regular price $76.00 USD
Regular price $95.00 USD Sale price $76.00 USD
MWCO
Filtration Area
Package
*Ships within: 3 Business Days
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Dimensions Effective membrane area 3.4cm²
Overall Length x Tube diameter 124.5 x 16.9 mm
Materials Membrane Material Regenerated Cellulose (RC)
Centrifuge Tube PP
Ultrafiltration device Polystyrene (GPPS)
Lid
High Density Polyethylene (HDPE)
Concentrator Capacity
Fixed Angle Rotor(45°) Swing Bucket Rotor
Maximum Sample Volume
4 mL
4 mL
Dead-stop volume
30 μL 40 μL
Final concentration volume 80-200μL
Centrifuge
Fixed Angle Rotor(45°) Swing Bucket Rotor
Maximum G-force
5000g 4000g
Sterility Delivery Condition
Nonsterile
Sterilization Method
Not Recommended
Package Pack Size 15pcs
Packaging Individually packed in PE bag


Cobetter BriScale® 4mL 10 kDa centrifugal filters are made of low-binding regenerated cellulose(RC) ultrafiltration membranes, which can achieve a high protein recovery rate >90%, using marker protein Ovalbumin(45 kDa) at 1 mg/mL, centrifuge 30 mins.

BriScale® centrifugal filter is ideal for the concentration of proteins and biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms. The vertical design and membrane surface area provide fast concentration time and rapid protein concentration for up to 80-fold concentration.

The centrifugal filter unit consists of a lid, a filter device and a centrifuge tube. Use a pipette to collect the concentrate from the filter device. The ultrafiltrate is collected in the centrifuge tube for further analysis.

BriScale® 4 RC Centrifugal Filters can handle samples up to 4 mL in rotors accepting 15 mL centrifuge tubes. Use in fixed angle 45°rotor(max starting volume of 4mL) or swing bucket rotor(max starting volume of 4mL).

Features

· 10 kDa MWCO RC membrane achieves a high recovery rate of>90%

· Color-coded cap with different MWCO, volume graduation for easy detection and user-friendly

· Low-binding regenerated cellulose(RC) ultrafiltration membranes

· Broad chemical compatibility pH 1-14

· 80-fold concentration with vertical membranes

· Translucent housing and volume marker make sample monitoring effortless.

Applications

 · Concentration and purification of nucleic acids, proteins such as antigens, antibodies, enzymes, etc and other biomolecules

· Desalting, buffer exchange and dialyzing proteins and antibodies for sample preparation

· Concentration of dilute or pre-purified proteins from chromatography

· Remove primers, adapters or molecular tags from the reaction mixture

· Purification of macromolecules in tissue culture extracts or cell lysates, and protein removal prior to HPLC and mass spectrometry.

How to choose Cobetter Centrifugal Filters?

To choose which type to use, determine the molecular weight (MW) of your macromolecule of interest, and select an ultrafiltration membrane with a molecular weight cut-off (MWCO) that is 2-3 times smaller than the MW of your macromolecule.

Filter MWCO Target MW
3 kDa 6 kDa < MW < 20 kDa
10 kDa 20 kDa < MW < 60 kDa
30 kDa 60 kDa < MW < 100 kDa
100 kDa 200 kDa < MW

Centrifugal Filter Units Structure & Adapted Equipment

Cobetter ultrafiltration centrifugal filter units consist of a lid, a filter unit, and a centrifuge tube.

It requires a rotor or rotor adapter compatible with 15mL (for 4mL centrifugal filter) or 50mL (for 15mL centrifugal filter) conical bottom centrifuge tubes. Both the swinging bucket rotor and the fixed-angle rotor must meet this requirement.

Cobetter ultrfiltration centrifugal filter structure

Processing Time

Processing time of different MWCO filters.

Protein Recovery Rate

The RC membrane and smooth inner wall design have extremely low protein adsorption. Can achieve a recovery rate of more than 90%.

Q: How to select a centrifugal filter?

A: First, determine the starting volume of your sample.

Second, determine the molecular weight (MW) of your macromolecule, then choose a centrifugal filter with a MWCO that is 2-3 times smaller than the MW of your macromolecule.

Q: Are Cobetter centrifuge filters reusable?

A: We recommend using them once, but some of our customers tried to use it for the same sample up to maximum 3 times, only for reference.

Q: How to clean Cobetter centrifuge filters?

A: First, soak them in 0.1M NaOH for 24 hours, then soak them in 20% ethanol for preservation.

Q: Can Cobetter centrifuge filters cut off 40,000 MW of pvp or 20-100nm nanosilver? If 20-100 nm nanosilver contains 40,000 MW pvp at the same time, can they be cut off at the same time?

A: You can choose 10kDa, and they can be cut off at the same time.

Q: Liposomes, want to be used to separate the free drug (MW 1550D), and the system solvent is PBS buffer.

A: Cobetter centrifuge filters are compatible, but we are not sure if the 5000xg centrifugal force will destroy the liposomes as we haven't tested it.

Q: There are two viruses that need to be concentrated, one with a diameter of 150nm~200nm and the other with a diameter of 40nm, which size of centrifuge filter is recommended?

A: You can choose 30kDa or 100kDa centrifuge filters.

Q: Which centrifuge filters are suitable for 100nm liposomes, or LNP? Will the recovery rate be low?

A: Use 100kDa centrifuge filters below 2,000 rpm. If the recovery rate is low please test the downstream filtrate to see if it's leaking through. 100kDa centrifuge filters shouldn't leak, so if a leak happens it's more likely due to excessive shear.

Q: Can Cobetter centrifuge filters be used to filtrate exosomes?

A: Yes, and it is recommended to use 100kDa first and then 3kDa centrifuge filters to concentrate exosomes with serum, and 3kDa centrifuge filters can be used directly for those without serum.

Q: What is the volume after concentration by 4ml centrifuge filters?

A: 80-200μL.

Q: What does KDa/Da mean?

A: KDa/Da is the molecular weight unit of proteins in biology.

Q: What's the material of the built-in ultrafiltration membrane?

A: Cobetter centrifugal filters are made with Regenerated Cellulose ultrafiltration membranes.

Q: What is the centrifuge time and speed for concentration to 100x?

A: Add 12mL solution upstream as an example, centrifuge at 3000~5000rpm until the upstream liquid remaining below the 250μL scale. Based on the feedback of our customers, centrifuge around 10~20 minutes, which is related to the concentration of the antigen.

Q: Do Cobetter centrifuge filters come with instructions?

A: Instruction manual is supplied with products.

Q: What is the size of the outer tubes?

A: The outer tubes for 4ml centrifuge filter is 15ml, and the outer tubes for 15ml centrifuge filter is 50ml.

Q: Is it possible to make liquid pass through the membrane by filtration rather than centrifugation?

A: No, it isn't. Cobetter centrifuge filters are compatible with centrifuges.

Q: Are Cobetter centrifuge filters compatible with regular centrifuges?

A: No, they aren't. They are matched to conical bottom centrifuges.

Q: Resistance of Cobetter centrifuge filter membrane.

A: You can check the chemical compatibility sheet in the product brochure which can be downloaded in the Documents Tab.

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