Low Extractable Nylon Filter Membrane Solve the Trouble of Interfering Peaks in HPLC

Low Extractable Nylon Filter Membrane Solve the Trouble of Interfering Peaks in HPLC


HPLC is a highly precise analytical instrument used for liquid sample analysis. Prior to HPLC analysis, it is crucial to filter the sample and mobile phase to remove insoluble particles. This helps prevent blockages in injection valves, tubing, and HPLC columns, while also reducing interference from impurities and improving data accuracy.

Figure 1: HPLC diagram

Figure 1: HPLC Diagram

Given the instrument's high analytical precision, the purity and extractable of the filter membrane are of utmost importance. The presence of extractable can lead to various issues, such as:

  • Impaired separation and integration of chromatographic peaks
  • Ghost peak formation
  • Alteration of stationary phase selectivity
  • Impact on chromatographic baseline

Decreased lifespan of high-pressure pump and other components (due to corrosive ions)

Figure 2: Interference peak

Figure 2: Interference Peak

Cobetter’s Low Extractable HPLC Nylon Membrane

Figure 3: Cobetter low extractable nylon nanofiber membrane SEM photograph

Figure 3: Cobetter Low Extractable Nylon Nanofiber Membrane SEM Photograph

As experts in the field of membrane applications, Cobetter continuously improves its membrane manufacturing process and has successfully developed a low extractable unsupported nylon nanofiber membrane for HPLC sample preparation, offering:

  • Improved detection results: Higher sensitivity and accuracy, suitable for trace analysis.
  • Reduced equipment wear: Prolongs the lifespan of chromatographic columns, reducing labor and equipment maintenance costs.

Fibers with diameters as small as 200nm, achieve high porosity and flow rate while ensuring retention efficiency.

Traditional HPLC Organic Phase Nylon Membrane

  • Phase transition process
  • Multiple solvents introduced during membrane manufacturing, making it hard to clean thoroughly
  • Prone to extractable contamination in organic solvents such as methanol and acetonitrile
  • Low porosity and flow rate

Cobetter Nylon Nanofiber Membrane

  • Electrospinning process (advanced technology from Japan)
  • Membrane manufacturing process free from any additives
  • Revolutionary advantages in clean background and low extractable
  • Fibers with diameters as small as 200nm, achieving high porosity and flow rate while ensuring retention efficiency

Typical Parameter Comparison


Product Name

Water Flow Rate mL/min@30kPa(Φ13mm)

Brand B

HPLC Nylon Filter-0.2μm


Brand A

HPLCNylon Filter-0.2μm



Nylon Nanofiber Filter-0.2μm



Figure 4: Dissolution test comparison

Figure 4: Dissolution Test Comparison


Cobetter's unsupported nylon nanofiber membrane demonstrates outstanding performance in HPLC dissolution testing. The amount of dissolution is significantly lower compared to brand A and brand B. When the retention time is greater than 5 minutes, the curve is smooth, without any noticeable dissolution peaks, and provides a clean background. It is highly suitable for sample preparation in HPLC.

Cobetter helps customers overcome application challenges and meet product requirements while reducing system costs through our exceptional products and continuous innovative solutions.